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Animal Breeding Techniques and Technologies PowerPoint Presentation

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Slide 1 - Animal Breeding Techniques and Technologies
Slide 2 - Embryo Transfer
Slide 3 - Embryo Transfer (ET) Why use ET? To make widespread use of females with high genetic merit: the female equivalent of AI. Naturally mated ewe: 1-3 lambs per year ET: up to 50 lambs can be produced per year Disadvantages Costly, labour intensive, requires technical expertise 14 Embryos ready for transfer What is ET? Fertilized embryos are removed from the uterus horn of a superovulated “donor” and transplanted into a synchronized “recipient” uterus
Slide 4 - ET Programme Progesterone CIDR Progesterone CIDR Mating (AI or natural) Recovery of embryos Embryos transplanted PMSG injection Remove CIDR GnRH injection DONOR (1) RECIPIENTS (6-14) 0 10 12 13 15 14 21 CIDR: controlled internal drug release PMSG: pregnant mare serum gonadotrophin GnRH: gonadotrophin releasing hormone FSH: follicle stimulating hormone
Slide 5 - Embryo collection in sheep
Slide 6 - Embryo collection in Cows
Slide 7 - ET Technique Donor is fasted for 12-24 hours Donor is prepared for surgery: anesthetized, put on surgical table, wool clipped, site sterilized Uterus and ovaries are exteriorised Corpus luteum are detected Eggs are recovered from uterus horns by flushing a sterile solution through with a foley catheter. Solution containing the embryos is collected in a watch glass Embryos are examined under microscope, only good quality embryos at correct stage of development are selected for transplant to the recipients
Slide 8 - Recipient is fasted for 12-24 hours Recipients are prepared for surgery: sedated, put in cradle, wool clipped, site sterilized, local anesthetic Abdomen is punctured with trocar, a laparoscope (light source) is inserted Corpus luteum are detected Forceps are inserted to exteriorise a uterus horn 1-2 embryos are implanted into the uterus horn with a pipette The embryo develops in the recipient as if it were her own ET Technique
Slide 9 - ET End Result
 

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