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Slide 1 - Tridax procumbence-A Potent Blood Coagulant Futane S.M., Mohite N.R., Nakat J.M. Shri. Shivaji college of Agri-Biotechnology, Amravati
Slide 2 - INTRODUCTION - Tridax procumbence is a widespread weed,commonly known as coat button in english,ekdandi or kambarmodi in marathi,ghamra in hindi and jayantiveda in sanskrit Botanical classification: Kingdom: Plantae Division: Magnoliophyta Class: Magnoliopsida Order: Asterales Family: Asteraceae Tribe: Heliantheae Genus: Tridax Species: T. Procumbens
Slide 3 - ABSTRACT Tridax procumbence is a species of flowering plant in a daisy family.It occurs in tropical as well as subtropical regions,in all the seasons. It exhibits antiseptic,insecticidal and hair growth promoting properties.More commonly the plant leaves are used to check haemorrhage from cuts,brucies,wounds. It also reported to have various pharmacological effects and antimicrobial activity against bacteria,hence prevent the infection and stimulate wound healing.
Slide 4 - KEYWORDS Tridax procumbence,blood coagulation,aspartic acid,marker enzymes,alanine transamylase.
Slide 5 - CHEMICAL COMPOSITION AND THEIR PHYSIOLOGICAL RESPONSE. Stigmasterol -antiinflametory property Betasitosterol-antiinflametory property Calcium-required for coagulation factors to bind to phospholipids. Phospholipids-required for tenase and prothrombinase to function. Marker enzymes –Helps in conversion of fibrinogen to fibrin. (aspartate,transaminase, lactate dehydrogenase, alkaline phosphatase)
Slide 6 - MECHANISM OF BLOOD COAGULATION Blood clotting comes under the concept of haemostasis.It is the major defence mechanism against the blood loss and occurs due to different substances mainly glycoproteins synthesized by the liver. It involves two pathways A)Extrinsic Pathway :It is the initial process in the clotting and involves factors that are not present in the blood. B)Intrinsic Pathway :It involves the series of reaction participated by the factors present in the blood. The two pathways are not independent but they are coupled together to convert protrombin to thrombin and fribrinogen to fibrin clot.
Slide 7 - Important factors responsible for blood coagulation. Fibrinogen:forms clot Prothrombin:it activates protein c and platelets. Tissue factor:co factor of prothrombin Calcium:helps to bind the coagulation factor to phospholipids. Christmas factor:forms tenase complex with antihaemophillic factors Hageman factor :activates plasma protein. High mol.Wt kininogen factor :support activation of hageman’s factor Staurt prower factor: forms prothrmbinase complex with labile factor Generation factor: precipitates in final common clotting pathway. Fibrin stabilizing factor: carried out cross links of fibrins.
Slide 8 - Diagram
Slide 9 - MATERIAL REQUIRED Sterile dry powder of Tridax procumbence,fresh blood sample without anticoagulant,test tubes,stopwatch,etc.
Slide 10 - METHOD Take 2 ml of fresh blood sample in two test tubes without adding any anticoagulant. Take one test tube as control and other as test sample. Add 200mg of sterile dry powder of Tridax procumbence in test sample. Do not add Tridax procumbence powder in control. Note coagulation time of both samples.
Slide 11 - OBSERVATION Test sample clots earlier than control.
Slide 12 - RESULT Dry powder of Tridax procumbence helps in faster blood coagulation
Slide 13 - CONCLUSION Tridax procumbence have great medicinal properties and can be used for – Wound healling Haemophillic disorders Bleeding disorders Antiinflamatory property Primary source of treatment in tribal areas where medical supervision is not available.
Slide 14 - References Dravya-gun vigyan-Dharmadhikari Textbook of pathology-harshmohan Textbook of biochemistry and human biology-J.P.Talwar Biochemistry-satyanarayan www.biologyforum.com www.sciencedictionary.com www.botanica.com
Slide 15 - Thank You.
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