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Slide 1 - Principles of genetic engineering L Mathias
Slide 2 - What is genetic engineering Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.
Slide 3 - Basic steps in genetic engineering Isolate the gene Insert it in a host using a vector Produce as many copies of the host as possible Separate and purify the product of the gene
Slide 4 - Step 1: Isolating the gene
Slide 5 - Step 1: Alternative method (using reverse transcriptase) Reverse transcriptase mRNA converted into cDNA Complementary strand produced using DNA polymerase Advantage – more mRNA in cell than DNA
Slide 6 - Step 2: Inserting gene into vector Vector – molecule of DNA which is used to carry a foreign gene into a host cell
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Slide 9 - Step 3: inserting vector into host See worksheet
Slide 10 - Replica plating
Slide 11 - Step 4: Multiplication of the host cells by cloning Large scale fermenters by cloning All genetically identical because of asexual reproduction
Slide 12 - Step 5: Extraction of desired gene product
Slide 13 - This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.