X

Download Cell And Tissue Culture PowerPoint Presentation

SlidesFinder-Advertising-Design.jpg

Login   OR  Register
X


Iframe embed code :



Presentation url :

Home / Health & Wellness / Health & Wellness Presentations / Cell And Tissue Culture PowerPoint Presentation

Cell And Tissue Culture PowerPoint Presentation

Ppt Presentation Embed Code   Zoom Ppt Presentation

PowerPoint is the world's most popular presentation software which can let you create professional Cell And Tissue Culture powerpoint presentation easily and in no time. This helps you give your presentation on Cell And Tissue Culture in a conference, a school lecture, a business proposal, in a webinar and business and professional representations.

The uploader spent his/her valuable time to create this Cell And Tissue Culture powerpoint presentation slides, to share his/her useful content with the world. This ppt presentation uploaded by dixitdeep12 in Health & Wellness ppt presentation category is available for free download,and can be used according to your industries like finance, marketing, education, health and many more.

About This Presentation

Cell And Tissue Culture Presentation Transcript

Slide 1 - Cell And Tissue Culture Mammalian Cells Advanced Higher Biology
Slide 2 - Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture
Slide 3 - What do you need to do it? Source of cell material -freshly prepared -stock of cell line -bacterial culture
Slide 4 - Suitable container Simple flask Sophisticated fermenter with computer-controlled monitoring
Slide 5 - Growth medium Glucose Water Amino acids Salts
Slide 6 - Opportunity for Gas Exchange Oxygen Carbon dioxide
Slide 7 - Animal serum Foetal Bovine Serum Essential for animal cell proliferation 5% - 10% of growth media
Slide 8 - Indicator Waste products causes change in pH Use indicator like phenol red Changes from red to yellow
Slide 9 - Control of Temperature and pH 37.5 OC pH 7.5
Slide 10 - Method for Measuring Cell Growth Counting cell numbers in culture (haemocytometer) Measure optical density in spectrophotometer
Slide 11 - Sterilisation Antibiotics Sterilisation
Slide 12 - Cells are either…. Anchorage – dependant Anchorage - independant
Slide 13 - Anchorage – independant cells Cells associated with body fluid -blood cells Grown in suspension Will eventually need subculturing
Slide 14 - Anchorage – dependant cells Most animal derived cells Adhere to bottom of a flask and form a monolayer Eventually cover entire surface of substratum (confluence) Proliferation then stops Need to subculture cells at this point (remove to fresh medium) Proliferation can begin again
Slide 15 - 2 main categories of animal cell cultures…. Primary culture Continuous cell line
Slide 16 - Primary Cultures Taken from fresh tissue Limited life span in culture Treated by proteolytic enzyme (Trypsin) Separate into single cells -epithelial cells -fibroblasts
Slide 17 - Continuous Cell Line Derived from humans Been transformed -lose sensitivity to factors associated with growth control Produce immortalised cell lines Cell lines are neoplastic Often lose their anchorage-dependence -associated with an altered xsome pattern More easily cultured
Slide 18 - ppt slide no 18 content not found
Slide 19 - Why do it? Particular cells can be isolated and cloned -isolation of mutant cells -investigate cell growth -produce hybrid cells that have applications in biotechnology Produce important pharmaceuticals -vaccines -hormones
Slide 20 - This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.