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Enteric Bacteria PowerPoint Presentation

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Published on : Mar 14, 2014
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Slide 1 - Enteric Bacteria Bacteria that cause diarrhea and dysentery
Slide 2 - Enteric Bacteria Enteric bacteria: secretory or watery diarrhea Vibrio cholerae ETECs (Enterotoxigenic E. coli) EPECs (Enteropathogenic E. coli) Invasive/Tissue Damaging Enteric pathogens: Bloody diarrhea and dysentery EIECs (Enteroinvasive E. coli) Shigella spp Salmonella spp EHECs (Enterohemmorhagic E. coli) Slow bacterial Infection Pathogens Helicobacter pylori
Slide 3 - What are enteric bacteria? Functional definition: Gram negative facultatively anaerobic rods Coliforms: enteric bacteria that ferment lactose to produce acid and gas
Slide 4 - What do gram negative bacteria look like? Lipopolysaccharide Cytoplasmic membrane Pili Outer membrane Flagella Periplasm
Slide 5 - Enteric Bacteria Enteric bacteria: secretory or watery diarrhea Vibrio cholerae ETECs (Enterotoxigenic E. coli) EPECs (Enteropathogenic E. coli) Comes from raw shellfish: These organisms (especially Vibrio) normally found in coastal regions Are consumed by small crustaceans and larger filter feeders Bacteria increase in number during conditions that produce algal bloom growth (seasonal or pollution)
Slide 6 - Enteric Bacteria Invasive/Tissue Damaging Enteric pathogens: Bloody diarrhea and dysentery EIECs (Enteroinvasive E. coli) Shigella spp (produces Shiga toxin) Salmonella spp-- found in undercooked chicken eggs and dairy products EHECs (Enterohemmorhagic E. coli)—found in undercooked hamburger etc produces Shiga toxin Example:: E. coli O157:H7
Slide 7 - Enteric Bacteria Slow bacterial Infection Pathogens Helicobacter pylori Causes gastric and duodenal ulcers ulcers are caused by localized non-specific immune response and by the fact that the bacteria produce cytotoxins Causes cancer Found associated with the pyloric region of the stomach where it colonizes stomach for decades and causes a persistant low grade inflammation of the stomach
Slide 8 - Helicobacter pylori—why doesn’t it get killed by stomach acids? H. pylori goes to region of stomach (pyloric region) where the pH of the stomach is relatively higher (It can sense pH and actively swim to the region that has a higher pH) H. pylori produces urease which breaks urea down into ammonia, this further increases the pH of the stomach
Slide 9 - Non-invasive bacteria that colonize the small intestines Vibrio cholerae and ETECs produces a toxin in the small intestines do not invade cells but reside on the surface of cells of the small intestines EPECS do not produce toxins changes the microvilli of the small intestines
Slide 10 - Vibrio cholerae and ETECs Makes contact with intestinal epithelial cells such they don’t get swept out by peristaltic movements of the intestines These bacteria have proteins at the end of their pili called adhesins The adhesins allow the bacteria to interact with glycoproteins and glycolipids on the surface of the intestinal epithelial cells Once established at cell surface the bacteria produce toxins that enter the cell and destroy the ionic balance of the cell
Slide 11 - Vibrio and ETEC toxins Vibrio cholerae produces cholera toxin and ETECS produce “cholera like toxin” Toxin has two components A subunit is actual toxin B subunit is delivery component The B subunits bind to epithelial cell surfaces and delivers the A subunit into the cell Once inside the cell the A subunit is activated and destroys ionic balance of cells
Slide 12 - EPECS do not produce toxin but produce enzymes that change the surface of the microvilli of the small intestinal epithelial cells. EPECS make contact with cells via bundle forming pili They then produce enzymes that are secreted to the inside of the cell The enzymes cause the actin underlying the microvilli to undergo actin rearrangements Such rearrangements lead to a pedestal formation at apical surface of cells This effaces the microvilli and prevents readsorption of water into the cell
Slide 13 - Invasive or tissue damaging Enteric bacteria Shigella dysenteriae, S. flexneri, S. boydii and S. sonnei EIECs EHECs Salmonella typhi, S. typhimurium
Slide 14 - EHECs EHECs cause hemorrhagic colitis and hemolyic uremic syndrome (HUS) HUS can cause renal failure We come in contact with EHECs when we eat undercooked hamburger—only 50 organisms required to cause disease or when the groundwater becomes contaminated near cattle farms EHECs are resistant to stomach acids EHECs travel to the colon where they attach to the surface of colonic epithelial cells and multiply
Slide 15 - EHECs EHECs attach to colonic epithelial cells via bundle forming pili They cause actin rearrangements that lead to pedestal formation and effacement of the brush border—(causes diarrhea) They produce a “Shiga like toxin” that enters cells and kills cells causes a massive immune response at site where bacteria are present together the cell’s death leads to a bloody, pus filled diarrhea
Slide 16 - EHECs, Shiga like toxin and HUS Shiga like toxin can travel in the blood stream where they reach the kidneys They kill kidney epithelial cells The dead cells plug up the small vessels in the kidneys leading to HUS (hemolytic uremic syndrome. This can lead to kidney failure
Slide 17 - Intracellular pathogens—Salmonella spp Salmonella typhimurium causes gastro-enteritis and a bloody diarrhea in humans S. typhimurium can be found in chickens, eggs, poultry products and milk Salmonella typhi causes typhoid fever and travels to the spleen, liver, kidneys and gallbladder Salmonella typhi can reside in the gallbladder of asymptomatic people, these people are carriers of the disease as they constantly shed the bacteria into the environment
Slide 18 - Typhoid Mary—case studies and reports